Jubei-chan the Ninja Girl: Secret of the Lovely Eyepatch

Jiyu Nanohana, a fourteen-year old junior highschool student who attends Hontsuru Jr.High unexpectedly meets Koinosuke Odago, an assistant of Yagyu Jubei who has searched 300 years for a worthy successor for the Lovely Eyepatch. He implores Jiyu to accept the Eyepatch but she steadfastly refuses.

The problem between Jiyu and Koinosuke continues as a new assassin arrives to eliminate Yagyu Jubei II.

The conflict continues when two new assassins arrive, takes the form of Jiyu's new teachers. They challenge her to a duel but is badly defeated, but Bantaro is injured during their fight.

Bantaro was cured by Shiro's mother. As things begin back to normal, Hajime appears and asks Shiro to join their clan; Shiro explains that he as no intentions of doing so, but Hajime reminds him that he knows Shiro has a longing to fight Jubei II and leaves. He then accompanies Jiyu and asks her about the Eyepatch, she says it is nothing and throws it away.

Mikage and her husband are requested to defeat Jubei II, they kidnap both Sai and Koinosuke in order to force Jiyu to give up the Eyepatch in exchange for the hostages. When Mikage puts on the Eyepatch, though, it starts burning her. Jiyu remembers that her mom felt hot before she died and instinctively jumps in and rips the Eyepatch off, leaving a smoky Mikage. Jiyu says that although they look similar, Mikage is not her mom. She transforms and defeat them.

In order to release Jiyu from the burden of becoming Yagyu Jubei and being targeted by the Ryujoji, Shiro challenges her to a duel. Later, Jiyu meets a Ryujoji assassin, he explains their 300-year old grudge and goal to her.